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| 1. |  | SALINAS, L.M.; BALSEIRO, A.; JIRÓN, W.; PERALTA, A.; MUÑÓZ, D.; FAJARDO, J.; GAYO, E.; MARTÍNEZ, I.Z.; RIET-CORREA, F.; GARDNER, D.R.; GARCÍA MARÍN, J.F. Neurological syndrome in goats associated with Ipomoea trifida and Ipomoea carnea containing calystegines. Toxicon, January 2019, Volume 157, Pages 8-11. Article history: Received 16 September 2018 // Received in revised form 7 November 2018 // Accepted 9 November 2018 // Available online 14 November 2018.
This work was partially supported by a grant from Instituto Nacional de...| Biblioteca(s): INIA Las Brujas. |
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| 3. | | Verdier, E.; Peralta, A.M.; Etchevers, A.; Maman, S.; Francis, M. Application of biotechnologies to plant quarantine and certification programs in Uruguay ln: Encuentro Latinoamericano de Biotecnología Vegetal, 2.; Encuentro Argentino de Biotecnología Vegetal, 3., 1995, Puerto Iguazú, Argentina REDBIO 95. Puerto Iguazú (Argentina): REDBIO, 1995. p. G.12| Biblioteca(s): INIA La Estanzuela. |
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| 4. | | Francis, M.; Verdier, R.; Etchevers, A.; Peralta, A.M. Citrus canker diagnostic using PCR: perspectives of application in Uruguay ln: Encuentro Latinoamericano de Biotecnología Vegetal, 2.; Encuentro Argentino de Biotecnología Vegetal, 3., 1995, Puerto Iguazú, Argentina REDBIO 95. Puerto Iguazú (Argentina): REDBIO, 1995. p. G.3| Biblioteca(s): INIA La Estanzuela. |
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| 5. | | Peralta, A. Unificación de procedimientos analíticos ln: Congreso Nacional de Ingeniería Agronómica, 6 : 1993 set 28-30 : Montevideo Trabajos presentados. Montevideo (Uruguay): Asociación de Ingenieros Agrónomos del Uruguay, 1993. pII. 13| Biblioteca(s): INIA Las Brujas. |
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| Registros recuperados : 16 | |
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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
01/10/2014 |
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Actualizado : |
09/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
A - 1 |
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Autor : |
ALQUEZAR, B.; RODRIGO, M.J.; LADO, J.; ZACARÍAS, L. |
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Afiliación : |
JOANNA LADO LINDNER, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
A comparative physiological and transcriptional study of carotenoid biosynthesis in white and red grapefruit (Citrus paradisi Macf.). |
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Fecha de publicación : |
2013 |
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Fuente / Imprenta : |
Tree Genetics & Genomes, 2013, v.9, no.5, p.1257-1269. |
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ISSN : |
1614-2942 |
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DOI : |
10.1007/s11295-013-0635-7 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 30 January 2013 / Revised: 26 March 2013 / Accepted: 15 April 2013 / Published online: 15 May 2013. |
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Contenido : |
ABSTRACT.
Accumulation of lycopene in citrus fruits is an unusual feature restricted to selected mutants. Grapefruit (Citrus paradisi Macf.) is the Citrus specie with greater number of red-fleshed mutants, but the molecular bases of this alteration are not fully understood. To gain knowledge into the mechanisms implicated in this alteration, we conducted a comparative analysis of carotenoid profile and of the expression of genes related to carotenoid biosynthesis and catabolism in flavedo and pulp of two grapefruit cultivars with marked differences in colouration: the white Marsh and the red Star Ruby. Mature green fruit of Marsh accumulated chloroplastic carotenoids, while mature tissues lacked carotenoids. However, accumulation of downstream products such as abscisic acid (ABA) and expression of its biosynthetic genes, 9-cis-epoxycarotenoid dioxygenase (NCED1 and NCED2), increased after the onset of colouration. In contrast, red grapefruit accumulated lycopene, phytoene and phytofluene, while ABA content and NCED gene expression were lower than in Marsh, suggesting a blockage in the carotenoid biosynthetic pathway. Expression analysis of three genes of the isoprenoid pathway and nine of the carotenoid biosynthetic pathway revealed virtually no differences in flavedo and pulp between both genotypes, except for the chromoplast-specific lycopene cyclase 2 (β-LCY2) which was lower in the pulp of the red grapefruit. The proportion in the expression of the allele with high (β-LCY2a) and low (β-LCY2b) activity was also similar in the pulp of both genotypes. Therefore, results suggest that reduced expression of β-LCY2 appears to be responsible of lycopene accumulation in the red Star Ruby grapefruit.
© 2013 Springer-Verlag Berlin Heidelberg. MenosABSTRACT.
Accumulation of lycopene in citrus fruits is an unusual feature restricted to selected mutants. Grapefruit (Citrus paradisi Macf.) is the Citrus specie with greater number of red-fleshed mutants, but the molecular bases of this alteration are not fully understood. To gain knowledge into the mechanisms implicated in this alteration, we conducted a comparative analysis of carotenoid profile and of the expression of genes related to carotenoid biosynthesis and catabolism in flavedo and pulp of two grapefruit cultivars with marked differences in colouration: the white Marsh and the red Star Ruby. Mature green fruit of Marsh accumulated chloroplastic carotenoids, while mature tissues lacked carotenoids. However, accumulation of downstream products such as abscisic acid (ABA) and expression of its biosynthetic genes, 9-cis-epoxycarotenoid dioxygenase (NCED1 and NCED2), increased after the onset of colouration. In contrast, red grapefruit accumulated lycopene, phytoene and phytofluene, while ABA content and NCED gene expression were lower than in Marsh, suggesting a blockage in the carotenoid biosynthetic pathway. Expression analysis of three genes of the isoprenoid pathway and nine of the carotenoid biosynthetic pathway revealed virtually no differences in flavedo and pulp between both genotypes, except for the chromoplast-specific lycopene cyclase 2 (β-LCY2) which was lower in the pulp of the red grapefruit. The proportion in the expression of the allele with high... Presentar Todo |
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Palabras claves : |
ABA; CAROTENOIDS; GENE EXPRESSION; GRAPEFRUIT; LYCOPENE. |
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Thesagro : |
CAROTENO; CITRUS. |
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Asunto categoría : |
F01 Cultivo |
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Marc : |
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022 $a1614-2942
024 7 $a10.1007/s11295-013-0635-7$2DOI
100 1 $aALQUEZAR, B.
245 $aA comparative physiological and transcriptional study of carotenoid biosynthesis in white and red grapefruit (Citrus paradisi Macf.).$h[electronic resource]
260 $c2013
500 $aArticle history: Received: 30 January 2013 / Revised: 26 March 2013 / Accepted: 15 April 2013 / Published online: 15 May 2013.
520 $aABSTRACT. Accumulation of lycopene in citrus fruits is an unusual feature restricted to selected mutants. Grapefruit (Citrus paradisi Macf.) is the Citrus specie with greater number of red-fleshed mutants, but the molecular bases of this alteration are not fully understood. To gain knowledge into the mechanisms implicated in this alteration, we conducted a comparative analysis of carotenoid profile and of the expression of genes related to carotenoid biosynthesis and catabolism in flavedo and pulp of two grapefruit cultivars with marked differences in colouration: the white Marsh and the red Star Ruby. Mature green fruit of Marsh accumulated chloroplastic carotenoids, while mature tissues lacked carotenoids. However, accumulation of downstream products such as abscisic acid (ABA) and expression of its biosynthetic genes, 9-cis-epoxycarotenoid dioxygenase (NCED1 and NCED2), increased after the onset of colouration. In contrast, red grapefruit accumulated lycopene, phytoene and phytofluene, while ABA content and NCED gene expression were lower than in Marsh, suggesting a blockage in the carotenoid biosynthetic pathway. Expression analysis of three genes of the isoprenoid pathway and nine of the carotenoid biosynthetic pathway revealed virtually no differences in flavedo and pulp between both genotypes, except for the chromoplast-specific lycopene cyclase 2 (β-LCY2) which was lower in the pulp of the red grapefruit. The proportion in the expression of the allele with high (β-LCY2a) and low (β-LCY2b) activity was also similar in the pulp of both genotypes. Therefore, results suggest that reduced expression of β-LCY2 appears to be responsible of lycopene accumulation in the red Star Ruby grapefruit. © 2013 Springer-Verlag Berlin Heidelberg.
650 $aCAROTENO
650 $aCITRUS
653 $aABA
653 $aCAROTENOIDS
653 $aGENE EXPRESSION
653 $aGRAPEFRUIT
653 $aLYCOPENE
700 1 $aRODRIGO, M.J.
700 1 $aLADO, J.
700 1 $aZACARÍAS, L.
773 $tTree Genetics & Genomes, 2013$gv.9, no.5, p.1257-1269.
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